CYTOGENETIC STUDIES
CHROMOSOME ANALYSIS

The Kleberg Cytogenetics Laboratory offers chromosome analysis on blood, amniotic fluid, chorionic villus, and tissue biopsy specimens.

Reasons for Referral:

Chromosome analysis is indicated for individuals with any of the following: multiple congenital anomalies, mental retardation, developmental delay, dysmorphic features, sexual ambiguity, multiple miscarriages, infertility, fetal demise, and gamete donor screening. For prenatal cases, chromosome analysis is indicated for advanced maternal age, family history of genetic disease or chromosome abnormality, abnormal maternal serum screening and abnormal ultrasound findings.

Testing Methodology:

Standard methodology is used for G-banded chromosome analysis and includes high-resolution analysis for all blood specimens.

Specimen Requirements:

Postnatal (whole blood in sodium heparin (green-top) tubes):
Newborn (< 3 months): Minimum 0.5 -2.0 CC
Adult/Child: Minimum 3.0 -5.0 CC

Prenatal:
Amnio: 20-30 cc direct fluid (discard first 2 cc collected) in a sterile conical centrifuge tube.
CVS: 15-20 mg chorionic villi in sterile tissue culture media

Tissue Biopsy:
Tissue: 3 mm biopsy in sterile media
Demise: samples of skin, cord, or villi in sterile media

Shipping and Handling:

All tubes must be labeled with the patient's testname and date of birth, and a completed requisition form must accompany each sample. If the laboratory is billing the patient's insurance, please enclose a copy of the insurance card. Ship specimens for overnight delivery at ambient temperature. Please notify the laboratory of incoming samples.

Turnaround Time:

Postnatal:
Newborn (< 3 months): 7-10 days
Adult/Child: 10-14 days

Prenatal:
Amnio: 7-10 days
CVS: 7-10 days

Tissue Biopsy:
Tissue: 14-21 days
Demise: 14-21 days

CPT Codes and Prices:

AFAFP: 82106
AchE: 82013
Blood: 88262, 88230, 88289, 88291, 88280
Chorionic Villus (CVS): 88267, 88235, 88280x2, 88285, 88291
Amniotic Fluid + AFP: 88269, 88235, 88280X2, 88285, 82106, 88291
Amniotic Fluid, No AFP: 88269, 88235, 88280x2, 88285
Cell Culture: 88233
Amniotic Fluid + AFP + AchE: 88269, 88235, 88280x2, 88285, 82106, 88291, 82013
Amniotic Fluid + AFP + AchE + FH: 88269, 88235, 88280x2, 88285, 82106, 88291, 82013, 83030

Forms:

>> Cytogenetics Requisition
>> Prenatal Requisition

Test Codes:

AFAFP: 8501
AchE: 8502
Blood: 8600
Chorionic Villus (CVS): 8700
Amniotic Fluid + AFP: 8500
Amniotic Fluid, No AFP: 8530
Cell Culture: 4530
Amniotic Fluid + AFP + AchE: 8510
Amniotic Fluid + AFP + AchE + FH: 8520